Modified Sirius Red Stain Kit（No Picric Acid）
Storage:RT, avoid light.
G1472-1016Manual
Product InformationRelated ProductsRelated PaperCOA
Product Information
| English Name | Modified Sirius Red Stain Kit（No Picric Acid） |
| Unit | Box |
| Storage | RT, avoid light. |
| Validity Period | 1 year |
| Specification | 2*50ml2*500ml |
Product Introduction:
Collagen fiber is the most widely distributed and abundant fiber in connective tissue. It is widely distributed in various organs. Type I collagen fibers are mainly bone, skin and tendon; Type Ⅱ collagen fibers are mainly cartilage collagen; Type Ⅲ collagen fibers are mainly found in embryonic tissues, adult blood vessels and gastrointestinal tract; Type Ⅳ collagen fibers are mainly in the basem*nt membrane. Sirius red and its lining dye solution are strong acid dyes, which are easy to combine with the basic groups in collagen molecules and have strong adsorption. Polarized light microscopy shows that the collagen fibers have the property of positive uniaxial birefringence. When combining with Sirius red compound staining solution, it could enhance the birefringence and improve the resolution, so as to distinguish the two types of collagen fibers.
Modified Sirius Red Stain Kit（No Picric Acid） is mainly composed of Iron Hematoxylin Stain and Sirius Red Stain, without picric acid. It is mainly used in the study of collagen fiber abnormality or fiber proliferation in various tissue diseases. Under the ordinary optical microscope, the collagen fibers of heart and blood vessels and other tissues are dyed red, which is helpful for the classification and grading of various fibrotic diseases under the polarized light microscope. Immunohistochemical technique can also display type Ⅰ and Ⅲ collagen fibers, but the antibodies used are expensive and time-consuming, while Sirius red staining reagent is cheap and easy to operate.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：
These protocols are for reference only. Solarbio does not independently validate thesemethods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
实验内容 肝癌石蜡切片-偏光显微镜结果胶原纤维在偏光镜下呈现双折光，Ⅰ型胶原纤维呈强橙黄色或亮红色，Ⅲ型胶原纤维呈绿色。
肝癌石蜡切片-光学显微镜结果胶原纤维呈红色，背景呈黄色，细胞核呈红褐色到黑色。
Related Products
Related Paper
HDAC9-mediated epithelial cell cycle arrest in G2/M contributes to kidney fibrosis in male mice
Click to check>>
Author：
Zhang Yang, Yang Yujie, Yang Fan, Liu Xiaohan, Zhan Ping, Wu Jichao, Wang Xiaojie, Wang Ziying, Tang Wei, Sun Yu, Zhang Yan, Xu Qianqian, Shang Jin, Zhen Junhui, Liu Min, Yi Fan
IF：
17.6940
Publish_to：
Nature Communications
PMID：
37230975
Construction of a Localized and Long-Acting CCN2 Delivery System on Percutaneous Ti Implant Surfaces for Enhanced Soft-Tissue Integration
Click to check>>
Author：
Minghao Zhou, Jing Wang, Jia Wang, Jingwei Yu, sh*tou Huang, Tengjiao Wang, Hongbo Wei
IF：
10.3830
Publish_to：
ACS Applied Materials & Interfaces
PMID：
37133335
MTHFD2 reprograms macrophage polarization by inhibiting PTEN
Click to check>>
Author：
Man Shang, Lina Ni, Xiao Shan, Yan Cui, Penghui Hu, Zemin Ji, Long Shen, Yanan Zhang, Jinxue Zhou, Bing Chen, Ting Wang, Qiujing Yu
IF：
9.9950
Publish_to：
Cell Reports
PMID：
37149861
Melatonin Ameliorates Hepatic Ferroptosis in NAFLD by Inhibiting ER Stress via the MT2/cAMP/PKA/IRE1 Signaling Pathway
Click to check>>
Author：
Qingyun Guan, Zixu Wang, Keyu Hu, Jing Cao, Yulan Dong, Yaoxing Chen
IF：
9.2000
Publish_to：
International Journal of Biological Sciences
PMID：
37564204
Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress
Click to check>>
Author：
Wenji Ren, Zixu Wang, Jing Cao, Yulan Dong, Tuanjie Wang, Yaoxing Chen
IF：
7.6750
Publish_to：
Antioxidants
PMID：
37237884
Extracellular matrix remodelling and stiffening contributes to tumorigenesis of salivary carcinoma ex pleomorphic adenoma——A study based on patient-derived organoids
Click to check>>
Author：
Chen Wanling, Gu Ting, Chen Qianqian, Qu Chuxiang, Zhang Chunye, Hu Yuhua, Xia Ronghui, Zhang Ying, Wang Min, Huang Xinyi, Li Jiang, Shi Chaoji, Tian Zhen
IF：
7.5000
Publish_to：
Cell and Bioscience
PMID：
37393249
Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea
Click to check>>
Author：
Xiaoxiao Yang, Zhigang Liu, Mengyuan Fang, Tingfeng Zou, Zhen Zhang, Xianshe Meng, Tianxiang Wang, Huawen Meng, Yuanli Chen, Yajun Duan, Qingshan Li
IF：
6.7000
Publish_to：
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
PMID：
37406380
Ginsenoside Rd,a natural production for attenuating fibrogenesis and inflammation in hepatic fibrosis by regulating the ERRα-mediated P2X7r pathway
Click to check>>
Author：
Long Cui, Yu-Jing Tan, Shi-Qi Xu, Bo-Feng Qin, Meng-Xue Xiu, Xue Zhang, Li-Qiang Shi, Hai-Ming Sun, Jian Song
IF：
6.3170
Publish_to：
Food & Function
PMID：
37249244
The Reduction of Uromodulin,Complement Factor H,and Their Interaction Is Associated with Acute Kidney Injury to Chronic Kidney Disease Transition in a Four-Time Cisplatin-Injected Rat Model
Click to check>>
Author：
Zheyu Xing, Kunjing Gong, Nan Hu, Yuqing Chen
IF：
6.2080
Publish_to：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
PMID：
37047611
Astragaloside IV Blunts Epithelial–Mesenchymal Transition and G2/M Arrest to Alleviate Renal Fibrosis via Regulating ALDH2-Mediated Autophagy
Click to check>>
Author：
Dong Li, Yuzhe Liu, Quancao Zhan, Yan Zeng, Ze Peng, Qifeng He, Qi Tan, Wenfu Cao, Shang Wang, Jianwei Wang
IF：
6.0000
Publish_to：
Cells
PMID：
37443810
Technical Q&A
Q&A（Total3 Users）
-
Anonymous user 请问冰冻切片可以做天狼星红染色吗2023-05-24 09:52:57
Administrator您好，可以做冰冻切片染色，冰冻切片通常较厚，可能需要增加染色时间，建议根据实际染色情况调整染色时间。
5分
- Anonymous user
请问用什么软件分析胶原1和3的定量呢2022-08-02 17:48:39
Administrator您好，用偏光显微镜观察可以定性观察区分，定量分析没有做过了，您如果实验涉及到这一方面再查一下相关文献吧
5分
- 王周健
改良版天狼星红染色可以用于肺纤维化染色吗？有在文献中验证过吗？天狼星红染色和Van Gieson染色有区别吗？2022-07-19 17:45:53
Administrator1. 本产品可用于各种组织病变时胶原纤维异常或纤维增生的染色。
2. 在配方上有所不同，Van Gieson氏染色液中含有苦味酸，本品中不含。经本品染色，胶原纤维在偏光镜下呈现双折光，Ⅰ型胶原纤维呈强橙黄色或亮红色，Ⅲ型胶原纤维呈绿色。5分
