Anti-CSNK2A1 antibody (ab236664) | Abcam (2024)

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Anti-CSNK2A1 antibody (ab236664) | Abcam (1)

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Overview

  • Product name

    Anti-CSNK2A1 antibody
    See all CSNK2A1 primary antibodies

  • Description

    Rabbit polyclonal to CSNK2A1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Xenopus laevisAnti-CSNK2A1 antibody (ab236664) | Abcam (2)

  • Immunogen

    Recombinant full length protein corresponding to Human CSNK2A1 aa 1 to the C-terminus.
    Database link: P68400

  • Positive control

    • WB: HeLa, HEK-293, HepG2, Raji and K562 whole cell lysates.IHC-P: Human brain and colon cancer tissues.ICC/IF: HepG2 cells.IP: HeLa whole cell lysate (ab150035).
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin 300
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)

  • Anti-CSNK2A1 antibody (ab236664) | Abcam (5)Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purity >95%.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Associated products

  • Compatible Secondaries

  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human CSNK2A1 protein (ab85981)
  • Related Products

    • Recombinant human CSNK2A1 protein (ab60763)
    • HEK-293 whole cell lysate (ab7902)
    • Recombinant Human CSNK2A1 protein (ab85981)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab236664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P

1/100 - 1/500.

WB (1)

1/500 - 1/5000. Predicted molecular weight: 45 kDa.

IP

1/200 - 1/2000.

ICC/IF

1/50 - 1/500.

Notes

IHC-P
1/100 - 1/500.

WB
1/500 - 1/5000. Predicted molecular weight: 45 kDa.

IP
1/200 - 1/2000.

ICC/IF
1/50 - 1/500.

Target

  • Function

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
    Contains 1 protein kinase domain.

  • Information by UniProt
  • Database links

  • Alternative names

    • Casein kinase 2 alpha 1 polypeptide antibody
    • Casein kinase 2 alpha antibody
    • Casein kinase II alpha 1 antibody

    see all

Images

  • Western blot - Anti-CSNK2A1 antibody (ab236664)

    All lanes : Anti-CSNK2A1 antibody (ab236664) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CSNK2A1 antibody (ab236664)

    HepG2 (human liver hepatocellular carcinoma cell line) cells labelingCSNK2A1 (green) using ab236664 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody (ab236664)

    Paraffin-embedded human colon cancer tissue stained for CSNK2A1 using ab236664 at 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody (ab236664)

    Paraffin-embedded human brain tissue stained forCSNK2A1 using ab236664 at 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • Immunoprecipitation - Anti-CSNK2A1 antibody (ab236664)

    CSNK2A1 was immunooprecipitated from 500 μg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab236664 at 1/200 dilution. For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).

    Lane 1: Rabbit control IgG instead of ab236664 in Hela whole cell lysate.
    Lane 2: ab236664 IP in HeLa whole cell lysate.
    Lane 3: 10 μg HeLa whole cell lysate (input)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

    Anti-CSNK2A1 antibody (ab236664) | Abcam (11)

  • Datasheet download

    Download

References (1)

Publishing research using ab236664? Please let us know so that we can cite the reference in this datasheet.

ab236664 has been referenced in 1 publication.

  • Wu W et al. S-Nitrosylation of G protein-coupled receptor kinase 6 and Casein kinase 2 alpha modulates their kinase activity toward alpha-synuclein phosphorylation in an animal model of Parkinson's disease. PLoS One 15:e0232019 (2020). PubMed: 32343709

Customer reviews and Q&As

Anti-CSNK2A1 antibody (ab236664) | Abcam (13)

Anti-CSNK2A1 antibody (ab236664) | Abcam (2024)

References

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